Glucoamylase/Pullulanase Blended Saccharification Enzyme For Starch Sugar
A glucoamylase / pullulanase compound enzyme for the production of
starch sugar, enables a greater conversion rate from starch to
glucose, suitable for the production of glucose with DX value above
1) Greater conversion rate from starch to glucose
2) Maximize utilization of raw materials
3) Can be used in the production of glucose with DX>96.5%
4) Stable activity ensures stable dosage rate, no need to adjust
dosage for each batch
HGA-101PU contains glucoamylase and pullulanase. Glucoamylase is
made from controlled fermentation of selected strains of
Aspergillus niger. Pullulanase is made from Bacillus licheniformis.
Glucoamylase can hydrolyze alpha-1,4 glucosidic linkages from the
non-reduction end of starches to release glucose. Pullulanase can
specifically cleave the α-1, 6 glycosidic bond of the branching
point of amylopectin, and cut out the entire branch structure to
form amylose. Thus it has higher conversion rate from starch to
HGA-101PU is mainly used for the production of starch sugar.
|Glucoamylase activity||100,000U/ml (minimum)|
|Pullulanase activity||800BU/ml (minimum)|
|Appearance||Yellow brown liquid|
|Product pH||3.0 to 5.0|
|Specific gravity||1.10 to 1.25 g/ml|
EFFECT OF pH AND TEMPERATURE ON ACTIVITY
The effective pH range for HGA-101PU is 3.0 to 5.5. For maximum
activity, the optimal pH range for HGA-101PU is 4.0 to 4.5, optimal
pH is 4.2.
See Figure 1.
Effective temperature range for HGA-101PU is 30 to 65℃, normal
temperature range is 55 to 65℃, optimal temperature is 60°C. It
will be inactivated above 65℃. Normally, this enzyme will be
completely inactivated if the saccharified liquid is boiled for 5
minutes at 80℃.
See Figure 2.
Add this product into the liquefied starches of substrates such as
corn, wheat, barley, tapioca, rice, potatoes, etc. can convert the
starches into glucose.
Recommended optimal temperature for industrial operations is 58 to
62°C, pH 4.0 to 4.5.
First, adjust pH, and then add this product. The dosage rate
depends on the raw materials and actual processing conditions. It
is recommended to conduct several trials to determine the actual
optimal dosage rate. A general recommended dosage rate is 0.35 to
0.6kg / TDS.
PACKAGING, STORAGE AND SHELF-LIFE
HGA-101PU is packaged in 25kg, 30kg food-grade HDPE drums or 1125
kg IBC totes. Customized packaging is available upon request.
Typical shelf-life is six months if it is stored below 25°C in its
original packaging, sealed and unopened, protected from sunlight.
Its shelf-life will be eighteen months if this product is stored
properly at 0 ∼10°C. Prolonged storage and/or adverse conditions
such as higher temperatures may lead to higher dosage requirement,
therefore should be avoided.